G5885

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

lyophilized powder, >= 550 units/mg protein (biuret)

• 동의어(들): G-6-P-DH
• CAS Number: 9001-40-5
• EC 번호: 1.1.1.49 (BRENDA, IUBMB)
• EC Number: 232-602-6
• MDL number: MFCD00081656
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bacterial (Leuconostoc mesenteroides)
• Quality Level: 300
• 유형: Type XXIV
• 양식: lyophilized powder
• 특이 활성도: >= 550 units/mg protein (biuret)
• 분자량: 128 kDa
• 구성: Protein, 15-40% biuret
• 응용 분야: agriculture
• 외래 활성: 6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%; PGI ≤0.01%
• 저장 온도: 2-8°C

설명

일반 설명

Glucose-6-Phophate Dehydrogenase (G-6-PDH) comprises His-Asp catalytic dyad and exists as a homodimer. Structurally, G-6-PDH encompasses a Rossmann dinucleotide binding fold in the coenzyme binding domain region. It also harbors a large β + α domain and has a unique aspartate residue at position 374.

애플리케이션

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples.

생화학적/생리학적 작용

Glucose-6-Phophate Dehydrogenase (G-6-PDH) can utilize either nicotinamide adenine dinucleotide phosphate (NADP⁺) or NAD⁺ as coenzyme making it crucial for bacterial metabolism.

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

단위 정의

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

물리적 형태

Lyophilized powder containing Ficoll and Tris buffer salts

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)