P5381

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)

Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)

• 동의어(들): D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
• CAS Number: 9001-41-6
• EC 번호: 5.3.1.9 (BRENDA, IUBMB)
• EC Number: 232-603-1
• MDL number: MFCD00131887
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bakers yeast
• Quality Level: 200
• 유형: Type III
• 양식: ammonium sulfate suspension
• 특이 활성도: ≥400 units/mg protein (biuret)
• 분자량: tetramer 119,500 Da±600
• 농도: ≥3 mg/mL protein Biuret
• 기술: activity assay: suitable
• 색상: white
• 단백질 ID 수납 번호: NP_009755.1
• UniProt 수납 번호: P12709
• 저장 온도: 2-8°C
• 유전자 정보: yeast ... PGI1(852495)

설명

일반 설명

Research area: Cell Signaling

Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.

애플리케이션

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:

• to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.
• as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.
• to convert fructose to glucose while evaluating fructose content in kudzu roots.

생화학적/생리학적 작용

Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP). It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.

단위 정의

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.

물리적 형태

Crystalline suspension in 2.6 M (NH₄)₂SO₄ containing 0.1 mM magnesium acetate, pH 7.0

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves