H2126

Hyaluronidase from sheep testes

Type II, lyophilized powder, ≥300 units/mg solid

• 동의어(들): Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• CAS Number: 37326-33-3
• EC 번호: 3.2.1.35 (BRENDA, IUBMB)
• EC Number: 253-464-3
• MDL number: MFCD00131351
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 결합: conjugate (Glucosaminoglycan)
• Quality Level: 200
• 유형: Type II
• 양식: lyophilized powder
• 특이 활성도: ≥300 units/mg solid
• 분자량: 55 kDa
• 저장 온도: −20°C

설명

일반 설명

Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.

애플리케이션

Hyaluronidase from sheep testes has been used:

• in flow cytometry
• in the isolation of putative spermatogonial stem cells (SSCs)
• to study the effects of magnetic stimulation in magnetic hydrogel properties
• in immunohistochemistry to visualize collagen type II distribution
• to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings
• for the degeneration of hydrogels

생화학적/생리학적 작용

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

단위 정의

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

물리적 형태

Lyophilized powder containing lactose

기타 정보

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)