I0408

Invertase Glycoprotein Standard

BioReagent, from Saccharomyces cerevisiae

• 동의어(들): Invertase from baker’s yeast (S. cerevisiae), β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase
• CAS Number: 9001-57-4
• EC 번호: 3.2.1.26 (BRENDA, IUBMB)
• EC Number: 232-615-7
• MDL number: MFCD00131410
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: Saccharomyces cerevisiae
• Quality Level: 200
• 제품 라인: BioReagent
• 양식: lyophilized powder
• 분자량: 60 kDa
• 농도: ≥0.5 mg/vial protein (E1%/280)
• 저장 온도: 2-8°C

설명

애플리케이션

Invertase Glycoprotein Standard has been used:

• to generate N-linked glycan library
• as a negative control to study the binding of lectins to high mannose structures
• for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis

The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.

Used in the production of confectionary foods and artificial honey.

생화학적/생리학적 작용

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

기타 정보

Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)