L2646

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-M, lyophilized powder

• 동의어(들): Phaseolus vulgaris agglutinin, PHA
• EC Number: 232-718-7
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• Quality Level: 300
• 양식: lyophilized powder
• 효능: <40 μg per mL agglutination activity
• 구성: Protein, 50-70% biuret
• 불순물: salt, free
• 저장 온도: 2-8°C

설명

생화학적/생리학적 작용

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

분석 메모

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable