11249738001

Phytohemagglutinin-L (PHA-L)

from Phaseolus vulgaris

• 동의어(들): PHA-L
• UNSPSC 코드: 23201100

속성

• Quality Level: 100
• 무균: non-sterile; 0.2 μm filtered
• 분석: >95% (SDS-PAGE)
• 양식: lyophilized (clear, colorless solution after reconstitution)
• 포장: pkg of 5 mg
• 제조업체/상표: Roche
• 응용 분야: hematology
• 저장 온도: 2-8°C

설명

일반 설명

PHA-L is purified from Phaseolus vulgaris (red kidney bean) by standard chromatographic techniques.
Phytohemagglutinin (PHA), the lectin extract from the red kidney bean, consists of only L-type subunits (isolectin L4, "leuko-agglutinin"). It is an N-acetylgalactosamine/galactose sugar-specific lectin. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.

애플리케이션

Phytohemagglutinin-L (PHA-L) consists of only L-type subunits (isolectin L₄, "leuko-agglutinin"), and is well suited for high-efficiency induction and the functional analysis of T-lymphocytes. It is highly purified and tested for high-efficiency stimulation of human lymphocytes. It is also used as positive controls for peripheral blood mononuclear cells (PBMC) stimulation.

생화학적/생리학적 작용

Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes.

규격

Biological activity: <5μg/ml for maximal stimulation of DNA synthesis (BrdU incorporation) with human peripheral blood lymphocytes

서열

PHA-L is a tetramer of 4 L-type subunits held together by noncovalent forces.

제조 메모

Working concentration: 1 to 5 μg/ml
Approximately 1 to 5 μg/ml for the stimulation of human peripheral blood lymphocytes
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable