L7759

Lectin from Arachis hypogaea (peanut)

peroxidase conjugate, lyophilized powder

• 동의어(들): PNA, Peanut agglutinin
• UNSPSC 코드: 12352202
• NACRES: NA.54

속성

• 생물학적 소스: Arachis hypogaea (peanut)
• Quality Level: 200
• 결합: peroxidase conjugate
• 양식: lyophilized powder
• peroxidase activity: ≥20 units/mg protein
• 기술: western blot: suitable
• 저장 온도: −20°C

설명

애플리케이션

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

생화학적/생리학적 작용

PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.

단위 정의

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.

물리적 형태

Contains sodium citrate

제조 메모

Prepared from peroxidase (P8375) using a modification of a published method, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)