L8146

Lectin from Ulex europaeus (gorse, furze)

peroxidase conjugate, lyophilized powder

• 동의어(들): Ulex europaeus agglutinin, UEA-I
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: Ulex europaeus
• Quality Level: 200
• 결합: peroxidase conjugate
• 분석: 70.00-90.00% (Lowry)
• 양식: lyophilized powder
• 효능: <40 μg/mL agglutination activity
• peroxidase activity: ≥15 units/mg protein
• 구성: Protein, ~80% Lowry
• 기술: western blot: suitable
• 저장 온도: 2-8°C

설명

애플리케이션

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

생화학적/생리학적 작용

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

포장

Package size based on protein content.

결합

Conjugates are prepared from affinity purified lectin.

단위 정의

One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.

물리적 형태

Contains sodium citrate buffer salts

제조 메모

Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.
Repurified by affinity chromatography after conjugation.

분석 메모

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)