L9420

Luciferase from Photinus pyralis (firefly)

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×10¹⁰ units/mg protein

• 동의어(들): Luciferase firefly
• CAS Number: 61970-00-1
• EC 번호: 1.13.12.7 (BRENDA, IUBMB)
• MDL number: MFCD00131546
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 분석: ≥98% (SDS-PAGE)
• 양식: buffered aqueous solution
• 특이 활성도: ≥10×10¹⁰ units/mg protein
• 분자량: 62 kDa
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.

애플리케이션

Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.

단위 정의

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.

Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of ¹⁴C in a PPO/POPOP cocktail.

물리적 형태

Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H302,H334,H373
• 예방조치 성명서: P260 - P264 - P270 - P284 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Resp. Sens. 1 - STOT RE 2 Oral
• 표적 기관: Kidney
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): 231.8 °F
• Flash Point (°C): 111 °C