M9004

Malic Dehydrogenase from bovine heart

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

• 동의어(들): L-Malate:NAD⁺ oxidoreductase, MDH
• EC 번호: 1.1.1.37 (BRENDA, IUBMB)
• EC Number: 232-622-5
• MDL number: MFCD00131567
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine heart
• Quality Level: 200
• 양식: ammonium sulfate suspension
• 특이 활성도: 2000-4000 units/mg protein (modified Warburg-Christian)
• 분자량: 35 kDa
• 불순물: ≤0.01% Glutamic-Oxalacetic Transaminase; ≤0.01% Glutamic-Pyruvic Transaminase
• 저장 온도: 2-8°C

설명

일반 설명

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

애플리케이션

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.

단위 정의

One unit will convert 1.0 μmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.

물리적 형태

Suspension in 3 M (NH₄)₂SO₄ - 0.01 M KH₂PO₄ solution, pH 7.3

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)