M9400

β-Mannosidase from Helix pomatia

5-30 units/mL, ammonium sulfate suspension, crude extract

• 동의어(들): β-D-Mannoside mannohydrolase
• CAS Number: 9025-43-8
• EC 번호: 3.2.1.25 (BRENDA, IUBMB)
• MDL number: MFCD00130638
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 양식: ammonium sulfate suspension
• Quality Level: 200
• 품질: crude extract
• 분자량: 94 kDa
• 농도: 5-30 units/mL
• 외래 활성: β-N-acetylglucosaminidase and α-mannosidase <1.0%
• 저장 온도: 2-8°C

설명

일반 설명

β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2.

애플리케이션

β-Mannosidase from Helix pomatia has been used:

• in the digestion of glycans for enzymatic analysis
• in the treatment of labeled monogalactosyl Manno-oligosaccharides
• in non-mammalian glycosidase inhibition assays

생화학적/생리학적 작용

β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements.

단위 정의

One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.

물리적 형태

Suspension in 3.0 M (NH₄)₂SO₄ containing 10 mM sodium acetate, pH approx. 4.0

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)