A2264

β-N-Acetylglucosaminidase from Canavalia ensiformis (Jack bean)

ammonium sulfate suspension, ≥10 units/mg protein

• 동의어(들): β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase, β-N-Acetylhexosaminidase
• CAS Number: 9012-33-3
• EC 번호: 3.2.1.52 (BRENDA, IUBMB)
• EC Number: 232-730-2
• MDL number: MFCD00081402
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Canavalia ensiformis
• Quality Level: 200
• 양식: ammonium sulfate suspension
• 특이 활성도: ≥10 units/mg protein
• 외래 활성: α- and β-galactosidase, and α-L-fucosidase ≤0.1%; α-mannosidase ≤0.2%
• 저장 온도: 2-8°C

설명

애플리케이션

β -N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer′s Disease . Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms .

생화학적/생리학적 작용

This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.

This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.

단위 정의

One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at pH 5.0 at 25 °C.

물리적 형태

Suspension in 2.5 M (NH₄)₂SO₄, pH 7.0

분석 메모

At pH 4.0, p-nitrophenyl β-N-acetylgalactosaminide is hydrolyzed at approximately 50% of the rate of hydrolysis of p-nitrophenyl β-N-acetylglucosaminide at pH 5.0.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable