모든 사진(3)

주요 문서

모든 문서 보기

MAK093

Phosphofructokinase (PFK) Activity Colorimetric Assay Kit

Name: Phosphofructokinase (PFK) Activity Colorimetric Assay Kit
Brand: SIGMA

sufficient for 100 colorimetric tests

동의어(들):

PFK Activity Assay Kit

로그인조직 및 계약 가격 보기

모든 사진(3)

About This Item

UNSPSC 코드:

12161503

NACRES:

NA.84

추천 제품

Slide 1 of 10

1 of 10

Supelco

FA20

Fructose Assay Kit

Sigma-Aldrich

T7638

Thaumatin from Thaumatococcus daniellii

Sigma-Aldrich

M7755

Monellin from Dioscoreophyllum cumminsii (serendipity berry)

Sigma-Aldrich

MAK015

Glucose-6-Phosphate Dehydrogenase Activity Assay Kit

Sigma-Aldrich

MAK476

Glucose Assay Kit

Sigma-Aldrich

MAK562

Branched Chain Amino Acid Kit

Supelco

GAHK20

Glucose (HK) Assay Kit

Sigma-Aldrich

MAK568

Lipid Peroxidation (MDA) Assay Kit

Sigma-Aldrich

P0294

Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle

Sigma-Aldrich

MAK329

L-Lactate Assay Kit

사용

sufficient for 100 colorimetric tests

검출 방법

colorimetric

저장 온도

−20°C

유전자 정보

human ... PFKL(5211), PFKM(5213), PFKP(5214)
mouse ... PFKL(18641), PFKM(18642), PFKP(56421)
rat ... PFKL(25741), PFKM(65152), PFKP(60416)

일반 설명

Phosphofructokinase (PFK) catalyzes the third step of glycolysis, the conversion of fructose-6-phosphate to fructose-1,6-diphosphate, the rate limiting step of glycolysis. PFK activity is highly regulated by multiple cofactors in addition to post-translational modifications and can be used to measure glycolytic flux in tissues. Deficiencies in PFK activity can result in the glycogen storage disease, glycogenosis type VII (Tarui′s disease), which is a glycogen storage disease.

애플리케이션

Phosphofructokinase (PFK) Activity Colorimetric Assay Kit has been used to measure the activity of phosphofructokinase.

적합성

Suitable for the measurement of phosphofructokinase activity, analysis of glucose metabolism and cell signaling and screening anti-cancer drugs in animal tissues (liver, brain, heart, muscles etc.) and cell culture (adherent or suspension cells).

원리

The Phosphofructokinase Colorimetric Assay kit provides a simple and direct procedure for measuring PFK activity in a variety of samples. PFK activity is determined by a coupled enzyme assay, in which fructose-6-phosphate and ATP is converted to fructose-1,6-diphosphate and ADP by PFK. The ADP is converted by the enzyme mix to AMP and NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the PFK activity present. One unit of PFK is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 7.4 at 37 °C.