MAK107

LCAT Activity Assay Kit

Supplied by Roar Biomedical, Inc.

• 동의어(들): Lecithin:cholesterol acyltransferase activity assay kit
• EC Number: 200-659-6
• UNSPSC 코드: 12161503
• NACRES: NA.84

속성

• 사용: sufficient for 100 fluorometric tests
• 응용 분야: pharmaceutical
• 검출 방법: fluorometric
• 관련 질환(들): cardiovascular diseases
• 저장 온도: −20°C
• 유전자 정보: human ... LCAT(3931)

설명

일반 설명

The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.

애플리케이션

Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.

LCAT Activity Assay Kit has been used for measuring lecithin-cholesterol acyl transferase activity in plasma samples.

특징 및 장점

Compatible with high-throughput handling systems.

적합성

Suitable for measuring phospholipase activity of LCAT in plasma or serum.

원리

The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λ_Ex=390 nm/λ_Em=470 nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.

분석 메모

Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.

키트 구성품 전용

• Substrate Reagent .1 mL
• READ Reagent 30 mL
• LCAT Assay Buffer 20 mL

안전성 정보

• 픽토그램: GHS02,GHS06,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H225,H301 + H311 + H331,H370
• 예방조치 성명서: P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
• Storage Class Code: 3 - Flammable liquids
• Flash Point (°F): 49.5 °F
• Flash Point (°C): 9.7 °C