N0513

Nerve Growth Factor-7S from murine submaxillary gland

lyophilized powder, suitable for cell culture

• 동의어(들): NGF-7S
• CAS Number: 93928-24-6
• EC Number: 210-123-3
• MDL number: MFCD00147703
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• Product Name: Nerve Growth Factor-7S from murine submaxillary gland, NGF-7S, lyophilized powder, suitable for cell culture
• 생물학적 소스: mouse
• 양식: lyophilized powder
• 효능: 2-250 ng/mL ED₅₀/EC₅₀
• 품질: endotoxin tested
• 분자량: protein 130 kDa
• 포장: pkg of 0.1 mg
• 기술: cell culture | mammalian: suitable
• 불순물: ≤10
• 색상: white
• UniProt 수납 번호: P01139
• 저장 온도: −20°C
• 유전자 정보: mouse ... Ngf(18049)

설명

일반 설명

Nerve growth factor (NGF) is a dimer of 118-residue polypeptide chains linked non-covalently. Both these polypeptides contain three intramolecular disulphide bridges. It forms the prototype of the neurotrophin growth factor family. In male mouse submandibular salivary gland, NGF is present in high amounts, and is present as a high molecular weight complex called nerve growth factor-7S (NGF-7S). NGF-7S is an α₂β₂γ₂ complex, where β₂-NGF is the active neurotrophin, and is linked to the dimers of the glandular kallikrein serine proteinases members, α-NGF and γ-NGF.

애플리케이션

Nerve Growth Factor-7S (NGF-7S) from murine submaxillary gland has been used for the treatment of following cells to induce neuronal differentiation-

• PC12
• Dorsal root ganglia (DRG) obtained from chick embryos of either sex (Gallus gallus)

생화학적/생리학적 작용

Nerve growth factor (NGF) is a neurotrophin, which facilitates the differentiation and survival of specific subtypes of neurons of central nervous system and peripheral nervous system. However, nerve growth factor-7S (NGF-7S) lacks any neurotrophic activity as most part of β-NGF subunit is buried within the NGF-7S complex.

물리적 형태

Lyophilized from 0.2 μm filtered solution in 25 mM sodium phosphate, pH 7.0.

제조 메모

Nerve growth factor-7S (NGF-7S) is isolated from male mouse submaxillary glands using a modification of the method of Varon, et al.

분석 메모

The biological activity is measured using a 3-day MTT assay.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)