N5415

Nerve Growth Factor-ß human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• 동의어(들): β-NGF, BNGF, NGF-β, NGF-beta
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• Product Name: Nerve Growth Factor-ß human, recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in HEK 293 cells
• 분석: ≥95% (SDS-PAGE)
• 양식: lyophilized powder
• 효능: ≤16 ng/mL ED₅₀/EC₅₀
• 분자량: protein calculated mol wt 13.5 kDa (Monomer)
• 포장: pkg of 10 μg; pkg of 100 μg
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1 EU/μg Endotoxin (β-NGF)
• UniProt 수납 번호: P01138
• 저장 온도: −20°C
• 유전자 정보: human ... NGF(4803)

설명

일반 설명

NGF-β gene is mapped to human chromosome 1p13.2.

Recombinant human β-NGF is expressed in human 293 cells as a homodimer (non-disulfide bonded) of 120 amino acids, with a calculated molecular weight of 13.5 kDa (monomer). Nerve growth factor β (β-NGF) is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. The production of natural β-NGF occurs in epithelial cells, and the pituitary gland.

생화학적/생리학적 작용

A mutation in the NGF-β gene is implicated in the loss of pain perception.

Nerve growth factor β (β-NGF) is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.

주의사항

The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at -20 °C.

물리적 형태

This product is lyophilized from PBS.

제조 메모

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile DDW containing 0.1% endotoxin-free recombinant human serum albumin, to a final concentration of 100 μg/ml.

저장 및 안정성

Store product at –20 °C. The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at –20 °C.

분석 메모

The specific activity is determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

면책조항

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable