N5661

Nuclease S₁ from Aspergillus oryzae

for single-strand DNA/RNA digestion

• 동의어(들): Endonuclease S₁
• CAS Number: 37288-25-8
• EC 번호: 3.1.30.1 (BRENDA, IUBMB)
• MDL number: MFCD00131010
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Aspergillus sp. (A. oryzae)
• Quality Level: 200
• 양식: solution
• 농도: ≥100000 units/mL
• 기술: DNA purification: suitable
• 적합성: suitable for nucleic acid purification
• 응용 분야: cell analysis
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

The Nuclease S1 enzyme from Aspergillus oryzae has the ability to degrade single-stranded oligonucleotides composed of either deoxynucleotides or ribonucleotides.

애플리케이션

Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA. It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.

생화학적/생리학적 작용

Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5′-phosphomononucleotide and 5′-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.

SI nuclease from Aspergillus oryzae can generate double-stranded DNA breaks in response to DNA nicks or abasic sites.

단위 정의

One unit will cause 1.0 microgram of single-stranded nucleic acid to become perchloric acid soluble per minute at pH 4.6 at 37°C.

물리적 형태

Solution containing 30 mM sodium acetate, 50 mM NaCl, 1 mM ZnCl₂, 50% glycerol, 2 mg/ml protein

키트 구성품 전용

• 30mM Sodium acetate .25-.25 %
• 50mM Sodium chloride .29 %
• 1mM Zinc chloride .01 %
• Glycerol 50 %
• 2mg/mL Protein .2 %

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable