O5500

Osmium tetroxide

Sealed ampule.

• 동의어(들): Osmium(VIII)-oxide, ‘Osmic acid’
• Linear Formula: OsO₄
• CAS Number: 20816-12-0
• Molecular Weight: 254.23
• EC Number: 244-058-7
• MDL number: MFCD00011150
• UNSPSC 코드: 12171500
• PubChem Substance ID: 57654490
• NACRES: NA.47

속성

• vapor density: 8.8 (vs air)
• Quality Level: 200
• vapor pressure: 7 mmHg ( 20 °C)
• 양식: powder
• 반응 적합성: reagent type: oxidant
• bp: 130 °C (lit.)
• mp: 39.5-41 °C (lit.)
• SMILES string: O=[Os](=O)(=O)=O
• InChI: 1S/4O.Os
• InChI key: VUVGYHUDAICLFK-UHFFFAOYSA-N

설명

일반 설명

Osmium tetroxide (OsO4) is a heavy tetrahedral, symmetrical molecule composed of osmium, a third-row transition metal. Osmium tetroxide is both a dye, and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium testroxide to ensure preservation of the lipids.

애플리케이션

Osmium tetroxide has been used as a fixative reagent for Brevibacterium mcbrellneri, and curcumin treated human lung cancer cell lines′ observation with a scanning electron microscope. It is also suitable as a fixative for ultrastructural analysis of Toxoplasma gondii′s skeletal muscle cells.

생화학적/생리학적 작용

Osmium tetroxide chemically combines with lipids making them insoluble; only 7% of the lipids can be extracted after the fixation. Therefore, the cell membranes comprising phospholipids become black and electron-dense after fixation in osmium tetroxide. It is also used to fix small amount of fat maintained in sections during paraffin processing.

기타 정보

Due to the hazardous nature of osmium tetroxide, it is recommended to use an ampule opener when using this chemical.

안전성 정보

• 픽토그램: GHS06,GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H300 + H310 + H330,H314
• 예방조치 성명서: P260 - P271 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Eye Dam. 1 - Skin Corr. 1B
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges