P1261

Phosphorylase a from rabbit muscle

lyophilized powder, 20-30 units/mg protein

• 동의어(들): 1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase
• CAS Number: 9032-10-4
• EC 번호: 2.4.1.1 (BRENDA, IUBMB)
• EC Number: 232-878-8
• MDL number: MFCD00167428
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: rabbit muscle
• Quality Level: 200
• 양식: lyophilized powder
• 특이 활성도: 20-30 units/mg protein
• 정제법: 2× crystallization
• 구성: Protein, 30-60% E1%/280
• 외래 활성: AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%; phosphoglucomutase ≤0.25%; phosphorylase b ≤30%
• 저장 온도: −20°C

설명

일반 설명

Phosphorylase A is the active form of glycogen phosphorylase which converts glycogen and orthophosphate (Pi) to glucose 1-phoshate (G-1-P).

애플리케이션

Phosphorylase a from rabbit muscle has been used to mix with glycogen for the preparation of limit dextrin. It has also been used to probe the array in oligosaccharide microarray analysis of phosphorylase activity.

Phosphorylase from rabbit muscle has been used in a study to assess the molecular mechanisms of oleanolic acid. It has also been used in a study to describe the conversion of phosphorylase B to A, through a conversion enzyme in the presence of ³²P-ATP.

생화학적/생리학적 작용

Dimeric phosphorylase b is converted to the more active tetramer, phosphorylase a, by the action of phosphorylase kinase.

Phosphorylase A can be inhibited by these compounds: Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls.

Phosphorylase participates in glycogenolysis. Deficiency of phosphorylase leads to several glycogen storage diseases with hepatomegaly.

단위 정의

One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate per min at pH 6.8 at 30 °C, measured in a system containing phosphoglucomutase, NADP, and glucose-6-phosphate dehydrogenase. (One μmolar unit is equivalent to ~45 Cori units.)

물리적 형태

Lyophilized powder containing β-glycerophosphate and EDTA

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)