P5400

Pectinesterase from orange peel

lyophilized powder, ≥150 units/mg protein

• 동의어(들): Pectin methylesterase, Pectin pectylhydrolase
• CAS Number: 9025-98-3
• EC 번호: 3.1.1.11 (BRENDA, IUBMB)
• EC Number: 232-807-0
• MDL number: MFCD00131812
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: ≥150 units/mg protein
• 구성: Protein, 20-50% biuret
• 환경친화적 대안 제품 특성: Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 환경친화적 대안 카테고리: , Enabling
• 저장 온도: 2-8°C

설명

일반 설명

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

애플리케이션

Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH₄)₂SO₄ and sodium chloride. It has been used to study the pectin methylesterase gene.

The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.

생화학적/생리학적 작용

Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.

단위 정의

One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.

물리적 형태

Contains (NH₄)₂SO₄ and sodium chloride

분석 메모

Protein determined by biuret.

기타 정보

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)