P6374

p53 human

recombinant, expressed in baculovirus infected Sf21 cells

• UNSPSC 코드: 12352202
• NACRES: NA.25

속성

• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in baculovirus infected Sf21 cells
• 분석: ≥90% (SDS-PAGE)
• 양식: aqueous solution
• 기술: dot blot: suitable
• UniProt 수납 번호: P04637
• 응용 분야: genomic analysis
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... TP53(7157)

설명

일반 설명

p53 protein is composed of an N-terminal domain with a transactivation domain and proline-rich region, a central core-DNA binding domain, and a C-terminal domain with tetramerization and regulatory domains. The TP53 gene which encodes for the p53 protein is mapped on the human chromosome at 17p13.1.

애플리케이션

Useful for the study of postranslational modification of p53, or protein-protein interaction studies.

p53 human has been used in dot blot.

생화학적/생리학적 작용

In addition, p53 was recently shown to induce differentiation of human embryonic stem cells. The effect is dependent on the DNA binding activity of p53.

p53 gene is highly conserved and expressed in normal tissues. It is the most commonly mutated gene in human cancer and more then 500 gene mutations have been described in various types of malignancies, hematologic as well as solid tumors. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells. Thus, p53 plays a vital role in suppressing the development of cancer.

p53 protein acts as a transcription factor that participates in regulating positively or negatively the expression of several responsive genes. It plays a role in regulating angiogenesis, cellular senescence, autophagy, cell survival, differentiation, and oxidative stress. Mutations in the TP53 gene lead to Li Fraumeni cancer syndrome.

물리적 특성

Histidine-tagged, full length human p53

물리적 형태

Supplied as a solution in 20 mM Tris-HCl, pH 8.0, 20% glycerol, 100 mM KCl, 0.2 mM EDTA, and 1 mM DTT.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable