SAB5900001

Alpaca Anti-GFP single domain antibody, agarose beads

recombinant Alpaca(VHH) Nano antibody agarose bead suspension

속성

• 생물학적 소스: alpaca
• Quality Level: 100
• 재조합: expressed in Pichia pastoris
• 결합: agarose conjugate
• 항체 생산 유형: primary antibodies
• 클론: recombinant monoclonal
• 양식: liquid
• 분자량: 15 kDa
• 기술: ChIP: suitable; RIP: suitable; co-immunoprecipitation (co-IP): suitable; immunoprecipitation (IP): suitable; mass spectrometry (MS): suitable
• 색상: colorless
• Matrix: (~45 um (cross-linked to 7.5% agarose beads))
• 동형: VHH
• 용량: binding capacity (10 μL slurry binds about 3ug-4 μg of recombinant GFP.)
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Fluorescent protein GFP exhibits bright green fluorescence when exposed to ultraviolet light and is frequently used as reporter and fusion tag. Anti-GFP single domain antibody magnetic beads are magnetic agarose spheres covalently coupled with VHH antibodies acquiring high specificity and affinity for GFP. These beads can efficiently capture and separate GFP and GFP tagged proteins, alongside with the associated proteins from cell extracts of mammal, plant, bacteria, yeast, insect, and other organisms.

특이성

Selectively recognizes GFP, mEGFP, superfolder GFP and most common CFP and YFP variants.

면역원

GFP Tagged Protein

특징 및 장점

• Anti Tag-Nano antibody beads are single-domain/variable region of heavy chain of camelid antibodies(sdAb/VHH).
• They are directly coupled to beads covalently.
• They have unique properties such as small size (~15kDa, 2-4nm) when compared to conventional antibodies.
• No heavy and light chains in downstream interactions.
• Acids and alkali resistance, tolerance to harsh conditions.
• Short incubation time 5 – 30 minutes.
• Low batch to batch variations.
• Typically, antibodies are not detectable in Western Blot.

포장

plastic tube

물리적 형태

1XPBS, 0.03% sodium azide, 50% glycerol.

기타 정보

Before usage, please invert the vial several times (DO NOT VORTEX) to form beads suspension, and take out beads’ suspension using a pipette tip with bigger opening (i.e., cut the tip of a 1000 μl pipette tip with sterile scissors). Once opened, it is recommended to use paraffin film to seal the cap.