SRP0360

PLK1 Polo Box Domain active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• 동의어(들): STPK13, polo-like kinase 1
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥90% (SDS-PAGE)
• 양식: aqueous solution
• 분자량: 53 kDa
• 포장: pkg of 100 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: 2.9 mg/mL
• NCBI 수납 번호: NM_005030
• UniProt 수납 번호: P53350
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PLK1(5347)

설명

일반 설명

Human Plk1 polo box domain (GenBank Accession No. NM_005030), amino acids 367-end with N-terminal GST-tag, MW=53 kDa, expressed in an E. coli expression system.

Polo-like kinase 1 (PLK1) belongs to the PLK family. It comprises polo-box domain (PBD). PBD, in turn, contains polo boxes (PB1 and PB2) with a 6-stranded β sandwich and an α helix as structural elements. The PLK1 gene is mapped to human chromosome 16p12.2.

애플리케이션

PLK1 Polo Box Domain active human has been used in the in vitro binding assay with various synthetic peptides coupled to streptavidin beads. It has also been used in binding studies with fluorescein-labeled substrate peptide 5-carboxyfluorescein-GPMQSpTPLNG to test the effect of various inhibitors.

생화학적/생리학적 작용

Polo-like kinase 1 (PLK1) is a mitotic kinase that mediates the regulation of various events in mitosis including chromosome segregation, cytokinesis, centrosome maturation, and separation. It activates cell division cycle 25 (CDC25) phosphatase which further regulates mitotic entry. Elevated expression of PLK1 is observed in many tumors.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable