SRP3106

LIGHT human

recombinant, expressed in Hi-5 Insect cells, ≥96% (SDS-PAGE), ≥96% (HPLC), suitable for cell culture

• 동의어(들): HVEM-L, TNFSF14
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in Hi-5 Insect cells
• 분석: ≥96% (HPLC); ≥96% (SDS-PAGE)
• 양식: lyophilized
• 분자량: 20.0-22.5 kDa
• 포장: pkg of 15 μg
• 기술: cell culture | mammalian: suitable
• 불순물: <0.1 EU/μg endotoxin, tested
• 색상: white to off-white
• UniProt 수납 번호: O43557
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... TNFSF14(8740)

설명

일반 설명

LIGHT belongs to the TNF family of ligands, and can signal through the herpes virus entry mediator type A receptor (HVEM, TNFRSF14), LTbR, or bind to a decoy receptor, DcR3. It is expressed in splenocytes, activated PBL, CD+8 tumor infiltrating lymphocytes, granulocytes, and monocytes. LIGHT has the ability to active NFkB, to co-stimulate the activation of lymphocytes and to induce apoptosis in certain human tumor cells. Recombinant human LIGHT has a calculated mass of 19.3 kDa containing 177 amino acid residues. Due to glycosylation LIGHT migrates between 20.0-22.5 kDa by SDS-PAGE under non-reducing conditions.

생화학적/생리학적 작용

LIGHT belongs to the TNF family of ligands, and can signal through the herpes virus entry mediator type A receptor (HVEM, TNFRSF14), LTbR, or bind to a decoy receptor, DcR3. Recombinant human LIGHT has a calculated mass of 19.3 kDa containing 177 amino acid residues. Due to glycosylation LIGHT migrates between 20.0-22.5 kDa by SDS-PAGE under non-reducing conditions.

서열

RLGEMVTRLP DGPAGSWEQL IQERRSHEVN PAAHLTGANS SLTGSGGPLL WETQLGLAFL RGLSYHDGAL VVTKAGYYYI YSKVQLGGVG CPLGLASTIT HGLYKRTPRY PEELELLVSQ QSPCGRATSS SRVWWDSSFL GGVVHLEAGE KVVVRVLDER LVRLRDGTRS YFGAFMV

물리적 형태

Lyophilized from 20 mM Sodium Phosphate, pH 7.0.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable