SRP5172

Catenin β, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 동의어(들): CTNNB, FLJ25606
• CAS Number: 847964-78-7
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in baculovirus infected Sf9 cells
• 분석: ≥70% (SDS-PAGE)
• 양식: buffered aqueous glycerol solution
• 분자량: ~115 kDa
• 기술: activity assay: suitable
• solubility: water: soluble
• 적합성: suitable for molecular biology
• NCBI 수납 번호: NM_001904
• 응용 분야: cell analysis
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... CTNNB1(1499)

설명

일반 설명

Research area: Apoptosis. β-catenins are cytoplasmic proteins that are ubiquitously expressed. These proteins are associated with E-cadherin at cellular junctions. CTNNB1 (catenin β-1) belongs to the armadillo family of proteins. This protein possesses a 130 amino acid N-terminal domain, 12 imperfect repeats of 42 amino acids termed arm repeats, and a 100 amino acid C-terminal domain. The gene encoding β-catenin is localized on human chromosome 3p22.1.

애플리케이션

Catenin β, GST tagged human has been used:

• as a component of protein kinase buffer for in vitro kinase assay
• in in vitro citrullination assay to characterize β-catenin citrullination
• to perform differentiation factor screening

생화학적/생리학적 작용

β-catenin interacts with T-cell factor and lymphoid enhancer-binding factor (TCF and LEF) transcription factors and is an essential member of the Wingless-Wnt signal transduction pathway. The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and glycogen synthase kinase 3 β (GSK3β), form a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of βcatenin by the proteasome. APC and the Siah-1 E3 ubiquitin-protein ligase-1 mediate a novel β-catenin degradation pathway linking p53 activation to cell cycle control. Activating mutations in the human β-catenin gene have been found in human colon cancer and melanomas. The protein has a role in homeostasis and development.

물리적 형태

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

제조 메모

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable