SRP6484

Carbonic Anhydrase 2/CA2 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• 동의어(들): CA-II, CAII, Car2
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in HEK 293 cells
• 태그: 6-His tagged (C-terminus)
• 분석: ≥95% (SDS-PAGE)
• 양식: lyophilized
• 분자량: calculated mol wt 30 kDa; observed mol wt 30 kDa (DTT-reduced. Ser 2 is the predicted N-terminal .)
• 포장: pkg of 10 μg; pkg of 50 μg
• 불순물: <1 EU/μg endotoxin (LAL test)
• UniProt 수납 번호: P00918
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... CA2(760)

설명

일반 설명

Carbonic Anhydrase 2 (CA2) is a zinc metalloenzyme and it helps in regulating acid-base balance in cells. It is a ubiquitous cytosolic protein. Carbonic anhydrase 2 also known as carbonate dehydratase II, carbonic anhydrase C, belongs to the class of a carbonic anhydrases. The associated gene is mapped to human chromosome 8q21.

애플리케이션

Carbonic Anhydrase 2/CA2 human has been used for CA2 ELISA (enzyme-linked immunosorbent assay).

생화학적/생리학적 작용

Carbonic Anhydrase 2 (CA2) catalyzes the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa). CA2 can bind to various transporters, such as anion exchanger 1 (AE1), NBCe1 (Na(+)/HCO3(-) cotransporter), NHE3 (Na(+)/H(+) exchanger 3) and MCT (monocarboxylate transporter). This association increases the rate of transport. Mutations in the CA2 gene are responsible for carbonic anhydrase II (CAII) deficiency syndrome, an autosomal recessive osteopetrosis characterised with renal tubular acidosis as well as cerebral calcifications. Changes in the expression of CA2 are linked with carcinogenesis.

물리적 형태

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH 8.0, with 150 mM NaCl, 1 mM DTT. Normally Mannitol or Trehalose is added as protectants before lyophilization.

재구성

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H319
• 예방조치 성명서: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable