T9285

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

• 동의어(들): Tris-EDTA wash buffer, TE buffer solution
• MDL number: MFCD00236359
• UNSPSC 코드: 41105319
• PubChem Substance ID: 329826904
• NACRES: NA.25

속성

• Grade: Molecular Biology
• Quality Level: 200
• 무균: 0.2 μm filtered
• 양식: liquid
• pH: 7.8-8.2 (1 × in water)
• 응용 분야: sample preparation
• 외래 활성: DNase, RNase, none detected
• 저장 온도: room temp
• SMILES string: NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
• InChI key: VLEIUWBSEKKKFX-UHFFFAOYSA-N

설명

일반 설명

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

애플리케이션

TE buffer has been used for:

• isolate, resuspend, or store DNA and RNA for PCR or qPCR
• gel electrophoresis, including Northern and Southern blotting
• bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
• antigen retrieval for immunohistochemistry

제조 메모

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

기타 정보

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안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable