RNASEA-RO

RNase A

from bovine pancreas

• 동의어(들): Rnase
• EC 번호: 3.1.27.5 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: bovine pancreas
• Quality Level: 100
• 양식: dry powder
• 특이 활성도: 50 U/mg (+25°C, RNA as substrate)
• 분자량: M_r = 13,700
• 포장: pkg of 100 mg (10109169001); pkg of 25 mg (10109142001)
• 제조업체/상표: Roche
• 파라미터: 65 °C optimum reaction temp.
• 기술: flow cytometry: suitable
• 최적 pH: 7.0-7.5
• 저장 온도: 2-8°C

설명

일반 설명

Bovine pancreatic RNase A is a member of the RNase A protein superfamily. It is one of the most characterized proteins, and is a kidney shaped basic protein. This protein is composed of 124 amino acids. In its native form, RNase A exists as a homodimer.

애플리케이션

• For analytical purposes
• Isolation of DNA (for this purpose, RNase A should be boiled)
• For cell cycle analysis by flow cytometry and propidium iodide (PI) staining

생화학적/생리학적 작용

RNase A catalyzes the transphosphorylation and degradation of RNA. It was the first protein identified to exhibit DNA melting functionality. This enzyme binds to several binding sites on single stranded (ss) RNA polynucleotide chain before degrading it. It interacts with ssDNA in a similar manner, which is responsible for its ‘DNA unwinding′ property. RNase A dimer obtained through tandemization shows toxicity to cancer cells.

단위 정의

One unit is the enzyme activity that causes a decrease in absorbance of A₀ to A₁ within 1 minute under assay conditions. A₀ to A₁ corresponds to the total conversion, A₁ being the final absorbance.

재구성

RNase A can be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, heated to 100 °C for 15 minutes to inactivate contaminating DNases and cooled slowly to room temperature and dispend into aliquots. Roche recommends subsequent storage at -15 to -25 °C.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable