U2383

Ubiquitin Carrier Protein H3/CDC34 human

≥95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain

• MDL number: MFCD03101856
• UNSPSC 코드: 12352202
• NACRES: NA.56

속성

• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in E. coli overproducing strain
• 분석: ≥95% (SDS-PAGE)
• 양식: solution
• 분자량: ~32 kDa
• UniProt 수납 번호: P49427; P61088
• 응용 분야: cell analysis
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... CDC34(997), UBE2N(7334)

설명

생화학적/생리학적 작용

Cdc34 is an essential gene for viability in yeast. Cdc34 mediates the transition from G₁ to S-phase of the cell cycle by degrading the S-phase cyclin/CDK inhibitor, SIC1 in yeast and p27(KIP1) in human. Cdc34 is also known to interact with the Skp1/Cdc53/F-box (SCF) ubiquitin ligase subunits Cul1 to degrade the NF-κB inhibitor IκBα in a phosphorylation-dependent manner.

Infected cell protein 0 of herpes simplex virus 1 was shown to bind UbcH3 and induce its degradation by promoting its polyubiquination. This resulted in increased expression of an UbcH3-target gene cyclin D1 and aided in further viral infection.

물리적 형태

Solution in 50 mM HEPES, 200 mM NaCl, 1 mM TCEP (pH 7.5), and 10% glycerol.

기타 정보

Manufactured for Sigma by Boston Biochem. Inc.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable