10107921001

Nuclease S7

Micrococcal nuclease, from Staphylococcus aureus

• 동의어(들): s7 nuclease
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: Staphylococcus aureus
• Quality Level: 100
• 양식: lyophilized
• 특이 활성도: ~15,000 U/mg
• 포장: pkg of 15,000 U
• 제조업체/상표: Roche
• 최적 pH: 7.5
• 배송 상태: wet ice

설명

애플리케이션

• Removal of endogenous RNA from in vitro translation systems
• RNA sequencing experiments

Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.

생화학적/생리학적 작용

Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca²⁺ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.

단위 정의

One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A₂₆₀.

저장 및 안정성

Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS07,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H315,H317,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash