콘텐츠로 건너뛰기
Merck
  • Effect of enzymatic pretreatment of various lignocellulosic substrates on production of phenolic compounds and biomethane potential.

Effect of enzymatic pretreatment of various lignocellulosic substrates on production of phenolic compounds and biomethane potential.

Bioresource technology (2015-06-22)
Michel Schroyen, Han Vervaeren, Hanne Vandepitte, Stijn W H Van Hulle, Katleen Raes
초록

Pretreatment of lignocellulosic biomass is necessary to enhance the hydrolysis, which is the rate-limiting step in biogas production. Laccase and versatile peroxidase are enzymes known to degrade lignin. Therefore, the impact of enzymatic pretreatment was studied on a variety of biomass. A significant higher release in total phenolic compounds (TPC) was observed, never reaching the inhibiting values for anaerobic digestion. The initial concentration of TPC was higher in the substrates containing more lignin, miscanthus and willow. The anaerobic digestion of these two substrates resulted in a significant lower biomethane production (68.8-141.7 Nl/kg VS). Other substrates, corn stover, flax, wheat straw and hemp reached higher biomethane potential values (BMP), between 241 and 288 Nl/kg VS. Ensilaged maize reached 449 Nl/kg VS, due to the ensilation process, which can be seen as a biological and acid pretreatment. A significant relation (R(2) = 0.89) was found between lignin content and BMP.

MATERIALS
Product Number
브랜드
제품 설명

Sigma-Aldrich
Sodium carbonate-12C, 99.9 atom % 12C
Sigma-Aldrich
trans-Ferulic acid, ≥99%
Sigma-Aldrich
4-Hydroxybenzoic acid, ReagentPlus®, ≥99%
Sigma-Aldrich
4-Hydroxybenzoic acid, ReagentPlus®, 99%
Sigma-Aldrich
Sodium malonate dibasic monohydrate, ≥98% (non-aqueous titration)
Sigma-Aldrich
trans-Ferulic acid, 99%
Sigma-Aldrich
Syringic acid, ≥95% (HPLC)
Sigma-Aldrich
Sodium malonate dibasic monohydrate, BioXtra
Sigma-Aldrich
Sodium malonate dibasic, ≥97.0% (NT)
Sigma-Aldrich
2-Hydroxycinnamic acid, predominantly trans, 97%
Sigma-Aldrich
Potassium dichromate, 99.98% trace metals basis
Sigma-Aldrich
Gallic acid, 97.5-102.5% (titration)
Sigma-Aldrich
Potassium phthalate monobasic, ACS reagent, acidimetric standard
Sigma-Aldrich
Potassium phthalate monobasic, puriss. p.a., ≥99.5% (T)
Sigma-Aldrich
Potassium phthalate monobasic, BioUltra, ≥99.5% (T)
Sigma-Aldrich
5α-Androstan-17β-ol-3-one, ≥97.5%
Sigma-Aldrich
5α-Androstan-17β-ol-3-one, purum, ≥99.0% (TLC)
Sigma-Aldrich
Methanol solution, suitable for NMR (reference standard), 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%
SAFC
Sodium chloride solution, 5 M
Sigma-Aldrich
5-(Hydroxymethyl)furfural, ≥99%, FG
Sigma-Aldrich
5-Hydroxymethyl-2-furaldehyde, 99%
Sigma-Aldrich
Hydrogen peroxide solution, 34.5-36.5%
Sigma-Aldrich
Acetic acid, ≥99.5%, FCC, FG
Sigma-Aldrich
Sodium chloride-35Cl, 99 atom % 35Cl
Sigma-Aldrich
Vanillic acid, ≥97%, FG
Sigma-Aldrich
Acetic acid-12C2, 99.9 atom % 12C
Sigma-Aldrich
Hydrogen peroxide solution, 30 % (w/w) in H2O, contains stabilizer
Sigma-Aldrich
Vanillic acid, 97%