Skip to Content
Merck

Key Documents

View All Documentation

Skip To

AB5731

Sigma-Aldrich

Anti-Nanog Antibody, NT

Chemicon®, from rabbit

Synonym(s):

Homeobox transcription factor Nanog, Nanog homeobox

Sign Into View Organizational & Contract Pricing

Select a Size

25 ML
₩666,145
50 ML
₩1,267,837

₩666,145

Please contact Customer Service for Availability

Select a Size

Change View
25 ML
₩666,145
50 ML
₩1,267,837

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

₩666,145

Please contact Customer Service for Availability
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SRE0023SRE0093G0876
β-Glucuronidase from abalone aqueous solution, β-glucuronidase ≥100,000 units/mL

SRE0037

β-Glucuronidase from abalone

β-Glucuronidase from abalone Purified, aqueous solution, β-glucuronidase 150,000-250,000 units/mL, β-glucuronidase ≥20,000,000 units/g protein

SRE0023

β-Glucuronidase from abalone

β-Glucuronidase from limpets (Patella vulgata) aqueous solution

SRE0093

β-Glucuronidase from limpets (Patella vulgata)

β-Glucuronidase from Helix pomatia Type H-2, aqueous solution, ≥85,000 units/mL

G0876

β-Glucuronidase from Helix pomatia

form

aqueous solution

form

aqueous solution

form

aqueous solution

form

aqueous solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

β-glucuronidase activity

≥100,000 units/mL

β-glucuronidase activity

≥20,000,000 units/g protein, 150,000-250,000 units/mL

β-glucuronidase activity

-

β-glucuronidase activity

-

secondary activity

≤8,000 units/mL sulfatase

secondary activity

≤7,500 units/mL sulfatase

secondary activity

-

secondary activity

≤7,500 units/mL sulfatase

General description

NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).

Specificity

Antibody recognizes Nanog.

Immunogen

Epitope: N-terminus
Synthetic peptide from mouse Nanog.

Application

Detect Nanog using this Anti-Nanog Antibody, N-terminus validated for use in WB.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Western blotting:
0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.

Quality

Routinely evaluated by Western Blot on Embryonic Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected NANOG on 10 μg of Embryonic Mouse Brain lysates

Target description

39 kDa (MW 34 kDa)

Physical form

Affinity purified
Purified rabbit polyclonal immunoglobulin in buffer containing liquid 0.1% sodium azide.

Storage and Stability

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Whole cell lysate from mouse embryonic stem cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

recommended

Product No.
Description
Pricing
  • Specification Sheet

    • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

    Select a Product Number

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Paul Awolade et al.
    European journal of medicinal chemistry, 187, 111921-111921 (2019-12-14)
    The emergence of disease and dearth of effective pharmacological agents on most therapeutic fronts, constitutes a major threat to global public health and man's existence. Consequently, this has created an exigency in the search for new drugs with improved clinical
    Miau-Guo Kang et al.
    Journal of analytical toxicology, 43(2), 96-103 (2018-10-03)
    Benzodiazepines is a large drug group used extensively to treat sleep disorders and anxiety; these drugs are frequently associated with various crimes such as murder and drug-facilitated sexual assault. With growing use and misuse of these compounds, confirmatory assays are
    Francesco Candeliere et al.
    Frontiers in microbiology, 13, 826994-826994 (2022-03-22)
    β-glucuronidases (GUS) of intestinal bacteria remove glucuronic acid from glucoronides, reversing phase II metabolism of the liver and affecting the level of active deconjugated metabolites deriving from drugs or xenobiotics. Two hundred seventy-nine non-redundant GUS sequences are known in the

    Articles

    Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

    β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service