H3136

Hematoxylin

certified by the BSC

• Synonym(s): Natural Black 1
• Empirical Formula (Hill Notation): C₁₆H₁₄O₆ · xH₂O
• CAS Number: 517-28-2
• Molecular Weight: 302.28 (anhydrous basis)
• Colour Index Number: 75290
• Beilstein: 91400
• EC Number: 208-237-3
• MDL number: MFCD00078111
• UNSPSC Code: 12171500
• PubChem Substance ID: 24895554
• NACRES: NA.47

Properties

• Agency: certified by the BSC
• Quality Level: 200
• form: powder
• mp: 200 °C (dec.) (lit.)
• solubility: ethanol: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O
• InChI: 1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1
• InChI key: WZUVPPKBWHMQCE-XJKSGUPXSA-N

Description

General description

Hematoxylin is a basophilic dye extracted from the tree Heamatoxylum campechianum. It specifically binds to acidic compounds and thus is useful for staining cell nuclei which contain acidic substances: DNA and RNA. Hematoxylin, when oxidized forms hematein, which has thick pale blue purple colour. Hematoxylin is often used along with eosin, in histological study under light microscope.[1]

Application

Hematoxylin has been used:

• to visualize anatomical features and to detect nucleus locus coeruleus adjacent to the periaqueductal gray region in brainstem samples[2]
• as a nuclear counterstain[3] to visualize the nucleus, adipocytes were counterstained with a hematoxylin reagent[4]
• to stain lung tissue for histological analysis[5]

Suitability

Certified for use in Weigert′s iron hematoxylin, Mayer′s hemalum, Harris hemalum and in Heidenhain′s technique

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves