D1821

DAF-FM

≥98% (HPLC)

• Synonym(s): 4-Amino-5-methylamino-2′,7′-difluorescein, Diaminofluorescein-FM
• Empirical Formula (Hill Notation): C₂₁H₁₄F₂N₂O₅
• CAS Number: 254109-20-1
• Molecular Weight: 412.34
• MDL number: MFCD29073049
• UNSPSC Code: 12352204
• PubChem Substance ID: 329798711
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: powder
• storage condition: desiccated
• color: white to brown
• solubility: DMSO: >1 mg/mL
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(O)cc4Oc5cc(O)c(F)cc35
• InChI: 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
• InChI key: DIJCILWNOLHJCG-UHFFFAOYSA-N

Description

Application

DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.

Biochem/physiol Actions

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO).

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

Caution

hygroscopic

Preparation Note

Color variation within the range from white to brown does not change performance.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable