E1032

Anti-EN-RAGE antibody produced in goat

affinity isolated antibody, lyophilized powder

• Synonym(s): Anti-CAGC, Anti-S100 calcium binding protein A12, Anti-S100A12, Anti-p6
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: goat
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): flow cytometry: suitable (0.25µg/10⁶ cells); immunohistochemistry: 5-15 μg/mL; western blot: 0.1-0.2 μg/mL
• UniProt accession no.: P80511
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... S100A12(6283)

Description

General description

EN-RAGE is a member of the S100/calgranulin superfamily.

Immunogen

purified, E. coli-derived recombinant human extracellular RAGE-binding protein.

Application

Anti-EN-RAGE antibody produced in goat is suitable for western blot analysis at a working concentration of 0.1–0.2μg/mL, immunohistochemistry at 5-15μg/mL and flow cytometry at 3-10μg/mL/10⁶ cells.

Biochem/physiol Actions

The gene encodes a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It stimulates innate immune cells via binding to receptor for advanced glycation end products (AGER), which activates the MAP-kinase and NF-κ-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. It also stimulates mast cell degranulation and activation which generates chemokines, histamine and cytokines with leukocyte recruitment to the sites of inflammation.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves