I7276

Anti-Insulin-Like Growth Factor-II antibody produced in goat

affinity isolated antibody, lyophilized powder

• Synonym(s): Anti-IGF-II
• MDL number: MFCD00162567
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: goat
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5-15 μg/mL using human Placenta; neutralization: 0.2-0.8 μg/mL; western blot: suitable
• UniProt accession no.: P01344
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... IGF2(3481)

Description

General description

Insulin-like growth factors -I and -II are important regulators of signalling that mediates growth and metabolism in cells. Insulin-like growth factor -II (IGF-II) is widely expressed during embryonic stages and regulates the placental development. IGF-II acts via specific receptor, IGF-IIR, the binding does not result in signal transduction, but is a mechanism to restrict the bioavailability of IGF-II. IGF-II also binds to IGF-IR which results in the activation of MAPK, Raf/Ras and PI3K cascades. The effects of IGF-II signalling are important in growth of blood vessels (angiogenesis) gene transcription, proliferation and transformation. Irregular IGF-II signaling has been implicated in many cancers including lung cancer, colo-rectal cancer, cervical cancer, testicular cancer, rhabdosarcoma, melanoma and hepatoblastoma
Anti-IGF-II recognizes human insulin-like growth factor II. It does not react with human IGF-I.

Specificity

The antibody will neutralize the biological activity of recombinant human IGF-II. It does not neutralize the biological activity of recombinant human IGF-I.

Immunogen

recombinant human IGF-II

Application

Anti- IGF-II antibody may be used for neutralization reactions, the ND50 of the antibody is 0.2-0.8 μg/ml. This antibody was used to neutralize IGF-II in glioblastoma cell lines. For immunohistochemistry a working antibody concentration of 5-15 μg/ml may be used for formalin-fixed, paraffin-embedded human placenta sections. The antibody is suitable for immunoblotting.

Physical form

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline, pH 7.4, with 5% trehalose

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves