I8904

Insulin-like Growth Factor-II from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

• Synonym(s): IGF-II
• MDL number: MFCD00241895
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Insulin-like Growth Factor-II from mouse, IGF-II, recombinant, expressed in E. coli, lyophilized powder
• biological source: mouse
• recombinant: expressed in E. coli
• Assay: >97% (SDS-PAGE)
• form: lyophilized powder
• quality: endotoxin tested
• mol wt: ~7.4 kDa
• packaging: pkg of 5X50 μg; pkg of 50 μg
• impurities: <1.0EUper1mg (LAL test)
• UniProt accession no.: P09535
• storage temp.: −20°C
• Gene Information: mouse ... IGF2(16002), Igf2(16002)

Description

General description

Recombinant Mouse IGF-II is produced from a DNA sequence encoding the mature IGF-II protein. Mouse IGF-II, a 67 amino acid protein, has a predicted molecular matrix of ~7.4 kDa. Mouse and human IGF-II share 91% sequence homology. Insulin-like growth factor II (also known as multiplication stimulating activity or MSA) and insulin-like growth factor I (IGF-I) belong to the family of insulin-like growth factors, which are structurally homologous to proinsulin. Mature IGF-I and IGF-II are highly conserved and share ~70% amino acid sequence identity. They have autocrine, paracrine, and endocrine functions.

Biochem/physiol Actions

Insulin-like growth factor II (IGF-II) is a potent mitogenic growth factor that mediates growth-promoting activities in embryonic development. IGF-II binds the IGF-II receptor with high affinity. IGF-I and IGF II are expressed in many tissues and cell types. IGF-II is mitogenic for a variety of cultured cells including human or chicken fibroblasts, mouse 3T3 cells, normal rat kidney.

Physical form

Lyophilized from a 0.2 μm filtered solution of 30% acetonitrile and 0.1% of TFA.

Analysis Note

The biological activity is measured in a serum-free cell proliferation assay using the human breast carcinoma cell line MCF-7.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves