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Key Documents

SRP0357

Sigma-Aldrich

NEK2 active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥65% (SDS-PAGE)

Synonym(s):

HsPK21, NEK2A, NIMA (never in mitosis gene a)-related kinase 2, NLK1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥65% (SDS-PAGE)

form

aqueous solution

mol wt

53 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

1.2 mg/mL

NCBI accession no.

NM_002497

UniProt accession no.

P51955

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... NEK2(4751)

General description

Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal HIS6-tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30°C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
4000920131

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