E2758

β-Estradiol

BioReagent, powder, suitable for cell culture

• Synonim(y): 1,3,5-Estratriene-3,17β-diol, 17β-Estradiol, 3,17β-Dihydroxy-1,3,5(10)-estratriene, Dihydrofolliculin
• Wzór empiryczny (zapis Hilla): C₁₈H₂₄O₂
• CAS Number: 50-28-2
• Masa cząsteczkowa: 272.38
• Beilstein: 1914275
• Numer WE: 200-023-8
• Numer MDL: MFCD00003693
• Kod UNSPSC: 12352209
• Identyfikator substancji w PubChem: 24894483
• NACRES: NA.77

Właściwości

• pochodzenie biologiczne: synthetic (organic)
• Poziom jakości: 200
• linia produktu: BioReagent
• Próba: ≥98% (HPLC)
• Formularz: powder
• metody: cell culture | mammalian: suitable; single cell analysis: suitable
• mp: 176-180 °C (lit.)
• rozpuszczalność: ethanol: 50 mg/mL, clear, colorless
• Warunki transportu: ambient
• temp. przechowywania: room temp
• ciąg SMILES: O[C@H]1CC[C@@]2([H])[C@]3([H])CCC4=CC(O)=CC=C4[C@@]3([H])CC[C@@]21C
• InChI: 1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17+,18+/m1/s1
• Klucz InChI: VOXZDWNPVJITMN-ZBRFXRBCSA-N
• informacje o genach: human ... ESR1(2099), ESR2(2100), ESRRA(2101), ESRRB(2103), GPER(2852), SERPINA6(866) ; mouse ... Esr1(13982), Esr2(13983), Esrra(26379) ; rat ... Afp(24177), Ar(24208), Esr1(24890), Esr2(25149), Shbg(24775)

Opis

Opis ogólny

β-Estradiol or estradiol-17 β (E2) is secreted majorly by the large preovulatory follicles in the ovary. In addition, estradiol can be synthesized de novo from cholesterol have androgens synthesized from cholesterol. The aromatization of androgen by enzyme aromatase (P450arom) results in the β-estradiol production.

Zastosowanie

β-Estradiol has been used:

• as a component of Dulbecco′s modified Eagle medium (DMEM) for culturing mammary tumor cells
• as a oestrogenic compound for testing neuroprotective effects
• as a component of medium199 for culturing oocytes

β-Estradiol is used to study cell differentiation and transformations (tumorigenicity).

Działania biochem./fizjol.

β-Estradiol (E2) is a major premenopausal hormone in women and is essential for spermiogenesis, sperm maturation and motility in men. Cultured bone marrow stem cells (BMSCs) have ability to synthesize E2 and could be exploited for treating estrogen deficiency. It mediates physiological functions through estrogen receptor (ER) α and ERβ.

The major estrogen secreted by the premenopausal ovary.

The major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female phenotype in embryogenesis and during puberty by regulating gene transcription and, thus, protein synthesis. It also induces the production of gonadotropins which, in turn, induce ovulation. Exposure to estradiol increases breast cancer incidence and proliferation.

Postać fizyczna

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Rekonstytucja

To prepare a 20 μg/ml stock solution, add 1 ml absolute ethanol to 1 mg β-estradiol; gently swirl to dissolve; add 49 ml sterile medium while mixing.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS09
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H351,H360FD,H362,H410
• Zwroty wskazujące środki ostrożności: P202 - P260 - P263 - P264 - P273 - P308 + P313
• Klasyfikacja zagrożeń: Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Lact. - Repr. 1A
• Kod klasy składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges