F0895

Human Fibronectin

from human plasma, liquid, 1 mg/mL, suitable for cell culture

• Synonim(y): Fibronectin
• Numer MDL: MFCD00131062
• Kod UNSPSC: 12352202
• eCl@ss: 42020141
• NACRES: NA.75

Właściwości

• Product Name: FIBRONECTIN FROM HUMAN PLASMA, liquid, 0.1% (Solution), BioReagent, suitable for cell culture
• pochodzenie biologiczne: human plasma
• Poziom jakości: 200
• sterylność: sterile
• linia produktu: BioReagent
• Formularz: liquid
• opakowanie: pkg of 1 mg; pkg of 2 mg; pkg of 5 mg
• stężenie: 0.1% (Solution)
• metody: cell culture | mammalian: suitable
• pokrycie powierzchni: 1‑5 μg/cm²
• rozpuszczalność: saline: 0.05 M (Tris-buffered)
• numer dostępu UniProt: P02751
• Specyficzność wiązania: Peptide Source: Collagen
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• informacje o genach: human ... FN1(2335)

Opis

Opis ogólny

Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. The FN1 gene mapped to human chromosome 2q35.
FN occurs as a dimeric protein, linked by two disulfide bonds at the end of C-terminal. Within the fibronectin structure, the domains are compactly arranged close to both the amino and carboxyl terminal of the peptide chains. Moreover, it has been observed that despite arising from a single gene up to 20 distinct variants of this gene have been identified in humans.

Zastosowanie

Fibronectin from human plasma has been used in the determination of H₂O₂ consumption rate. It has also been used in coating the Sylgard^® models for cell culture. It has also been used in cell adhesion and to directly pattern the protein onto the polymeric film.

Działania biochem./fizjol.

Fibronectin function encompasses a diverse range of biological processes including cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Fibronectin protein plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Mutations in FN1 results in glomerulopathy.

Cechy i korzyści

• Adhesive glycoprotein
• Derived from human plasma
• Recommended surface coverage of 1-5 μg/cm2

Uwaga dotycząca przygotowania

This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C

Komentarz do analizy

Protein homogeneity is evaluated by immunoelectrophoresis.

Informacje prawne

Sylgard is a registered trademark of The Dow Chemical Company or an affiliated company of Dow

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable