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Key Documents

05-143

Sigma-Aldrich

Anti-PLA2 Antibody, secretory

Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

mouse

Quality Level

antibody form

purified antibody

clone

monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

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This Item
07-62307-169-ISAB4200757
biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

mouse

species reactivity

human

species reactivity

rat, mouse

species reactivity

human

species reactivity

human

clone

monoclonal

clone

polyclonal

clone

polyclonal

clone

12-6-5, monoclonal

antibody form

purified antibody

antibody form

serum

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

technique(s)

ELISA: suitable, western blot: suitable, immunohistochemistry: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

flow cytometry: suitable, immunoblotting: 1-2 μg/mL using partial recombinant human PLA2R1 protein (predicted ~150kDa), immunofluorescence: suitable, immunohistochemistry: suitable

UniProt accession no.

P14555

UniProt accession no.

P11684

UniProt accession no.

O60733

UniProt accession no.

Q13018

Specificity

human type II secretory PLA2 in sperm, disc and synovial fluid

Immunogen

Purified human PLA2; recognizes type II (secretory) PLA2.

Application

Research Category
Signaling
Research Sub Category
Lipid Signaling
This Anti-PLA2 Antibody, secretory is validated for use in ELISA, IH, WB for the detection of PLA2.

Quality

routinely evaluated on PLA2 under non-reducing conditions

Target description

14/18 kDa

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide
DEAE cellulose chromatography
Format: Purified

Storage and Stability

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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    G Dorsam et al.
    Clinical chemistry, 41(6 Pt 1), 862-866 (1995-06-01)
    Previously we reported that uremic plasma contained eight times more phospholipase A2 (PLA2) activity than control plasma (Costello et al., Clin Chem 1990;36:198-200). That study, however, did not distinguish between various PLA2s that could contribute to the observed increase. Therefore
    Angiotensin II type 2 receptor stimulation of neuronal delayed-rectifier potassium current involves phospholipase A2 and arachidonic acid
    Zhu, M., et al
    The Journal of Neuroscience, 18, 679-686 (1998)
    X D Qu et al.
    Infection and immunity, 66(6), 2791-2797 (1998-05-29)
    We examined human tears for molecules that killed gram-positive bacteria. The principal mediator of bactericidal activity against staphylococci proved to be a calcium-dependent enzyme, secretory phospholipase A2. Whereas the concentration of secretory phospholipase A2 in the normal tear film exceeded
    Detection of natural peptide antibiotics in human nasolacrimal ducts.
    F P Paulsen, T Pufe, U Schaudig, J Held-Feindt, J Lehmann, J M Schroder, B N Tillmann
    Investigative Ophthalmology & Visual Science null
    Yan Fu et al.
    Journal of neuroscience research, 85(13), 2870-2881 (2007-06-07)
    Coherent anti-Stokes Raman scattering (CARS) microscopy, which allows vibrational imaging of myelin sheath in its natural state, was applied to characterize lysophosphatidylcholine (lyso-PtdCho)-induced myelin degradation in tissues and in vivo. After the injection of lyso-PtdCho into ex vivo spinal tissues

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