Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

71238-M

Sigma-Aldrich

pIEx-2 DNA - Novagen

Novagen′s pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera derived insect cells.

Synonym(s):

InsectDirect™ System- pIEx-2 DNA

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106611
NACRES:
NA.51
Pricing and availability is not currently available.

Quality Level

100

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SRP0643SRP0641SRP0635
PD-L1 (D26A), FC Fusion, Biotin Labeled recombinant, expressed in HEK 293 cells

SRP0633

PD-L1 (D26A), FC Fusion, Biotin Labeled

PD-L1 (K124A), FC Fusion, Biotin Labeled recombinant, expressed in HEK 293 cells

SRP0643

PD-L1 (K124A), FC Fusion, Biotin Labeled

PD-L1 (D122A), FC Fusion, Biotin Labeled recombinant, expressed in HEK 293 cells

SRP0641

PD-L1 (D122A), FC Fusion, Biotin Labeled

PD-L1 (Y56A), FC Fusion, Biotin Labeled recombinant, expressed in HEK 293 cells

SRP0635

PD-L1 (Y56A), FC Fusion, Biotin Labeled

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

mol wt

54 kDa

mol wt

54 kDa

mol wt

54 kDa

mol wt

54 kDa

form

frozen liquid

form

frozen liquid

form

frozen liquid

form

frozen liquid

Gene Information

human ... CD274(29126)

Gene Information

human ... CD274(29126)

Gene Information

human ... CD274(29126)

Gene Information

human ... CD274(29126)

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

The pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-2 contains N-terminal GST•Tag, His•Tag, and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
pIEx-2 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

For sequencing or PCR of recombinants in pIEx-2, refer to the table below to choose the appropriate primers.

Warning

Toxicity: Standard Handling (A)

Legal Information

INSECTDIRECT is a trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

  • Specification Sheet

    • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Jennifer L Warner-Schmidt et al.
    Biological psychiatry, 68(6), 528-535 (2010-07-02)
    The protein p11 (also called S100A10) is downregulated in human and rodent depressive-like states. Considerable experimental evidence also implicates p11 in the mechanism of action of antidepressant drugs and electroconvulsive seizures, in part due to its interaction with specific serotonin
    Per Svenningsson et al.
    Nature reviews. Neuroscience, 14(10), 673-680 (2013-09-05)
    Studies of the multifunctional protein p11 (also known as S100A10) are shedding light on the molecular and cellular mechanisms underlying depression. Here, we review data implicating p11 in both the amplification of serotonergic signalling and the regulation of gene transcription.
    p11 and its role in depression and therapeutic responses to antidepressants
    Svenningsson P, et al.
    Nature Reviews. Neuroscience, 14(10), 673-680 (2013)
    Disruption of annexin II/p11 interaction suppresses leukemia cell binding, homing and engraftment, and sensitizes the leukemia cells to chemotherapy
    Gopalakrishnapillai A, et al.
    Testing, 10(10), e0140564-e0140564 (2015)
    Jiqiang Guo et al.
    Scientific reports, 6, 17029-17029 (2016-01-30)
    Depression is one of the major side effects of interferon alpha (IFN-α) treatment, but the molecular mechanism underlying IFN-α-induced depression remains unclear. Several studies have shown that the serotonin receptors 5-HTR1b and 5-HTR4 play key roles in the anti-depression effects
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service