AB6065

Anti-Fibulin-5 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Fibulin-5, FIBL-5, Developmental arteries and neural crest EGF-like protein, Dance, Urine p50 protein, UP50
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse, rat
• species reactivity (predicted by homology): rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_006320
• UniProt accession no.: Q9UBX5
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... FBLN5(10516)

Description

General description

Fibulin-5 (also known as EVEC/DANCE) is an extracellular matrix protein. Fibulin-5 is most commonly found in blood vessels and cardiac valves during embryogenesis and in adult tissue that contains elastic fibers like the skin, lungs, uterus and aorta. Fibulin-5 travels to the surface of the elastic fibers and its activity is dependent on calcium. Fibulin-5 seems to work as a scaffold protein that facilitates the binding of the elastic fibers to cells and the formation of the elastic fibers themselves. Mutations of fibulin-5 have been associated with age-related macular degeneration (AMD) and cutis laxa (CL).

Specificity

This antibody recognizes Fibulin-5 near the C-terminus.

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human Fibulin-5 near the C-terminus.

Application

Anti-Fibulin-5 Antibody detects level of Fibulin-5 & has been published & validated for use in WB.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Quality

Evaluated by Western Blot in mouse heart tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Fibulin-5 in 10 µg of mouse heart tissue lysate.

Target description

~54 kDa observed.
The calculated molecular weight is 50 kDa; however bands have been observed at ~56 kDa (Freeman, L.J., et al. (2005) Biochem. J. 388:1-5).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse heart tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable