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Key Documents

AB9900

Sigma-Aldrich

Anti-IDO Antibody

Chemicon®, from rabbit

Synonym(s):

Indoleamine 2,3-dioxygenase

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

NM_002164.4

UniProt accession no.

P14902

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form

waxy solid

form

powder

form

solid

form

solid

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100, 200

mol wt

3,000-3,700

mol wt

average mol wt 3,350

mol wt

Mr 2700-3300

mol wt

Mr 950-1050

impurities

endotoxin, tested

impurities

≤0.01% Phosphorus (P), ≤0.1% Insoluble matter

impurities

insoluble matter, passes filter test, ≤0.001% peroxides (as H2O2)

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

biological source

synthetic (organic)

biological source

-

biological source

-

biological source

-

grade

Hybri-Max™

grade

-

grade

-

grade

for molecular biology

Specificity

IDO [Indoleamine 2,3-dioxygenase], mouse.

Immunogen

Recombinant mouse IDO.

Application

Anti-IDO Antibody detects level of IDO & has been published & validated for use in ELISA & WB.
Western blot: 1:2,000-1:5,000 using ECL. Reacts with the ~45 kDa IDO protein. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature.

ELISA, direct and indirect: 1:2,000-1:10,000.

Optimal working dilutions must be determined by the end user.

Target description

45 kDa

Linkage

Replaces: 04-1056

Physical form

Format: Purified
Liquid on PBS, pH 7.4.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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