MABS1288

Anti-VLDLR Antibody, clone 4C4G5

clone 4C4G5, from rat

• Synonym(s): Very low-density lipoprotein receptor, VLDL receptor, VLDL-R, VLDLR
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 4C4G5, monoclonal
• species reactivity: mouse
• technique(s): immunofluorescence: suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001154892
• UniProt accession no.: P98156
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: mouse ... Vldlr(22359)

Description

General description

Very low-density lipoprotein receptor (UniProt P98156; also known as VLDL receptor, VLDL-R) is encoded by the Vldlr gene (Gene ID 22359) in murine species. Murine very-low-density lipoprotein (VLDL) receptor (VLDLR) is an 110-kDa type I membrane receptor initially produced with a signal peptide sequence (a.a.1-27). The mature receptor has a large extracellular region (a.a. 28-797), a transmembrane domain (a.a. 798-819), and a cytoplasmic tail (a.a. 820-873). VLDLR belongs to the LDLR family of membrane glycoproteins whose members also inculde the low-density lipoprotein receptor (LDLR), the LDLR-related protein (LRP), ApoER2 (LRP-8), and Megalin (LRP-2). The overall extracellular structural features are similar among all LDLR family members. VLDLR contains a cluster of eight cysteine-rich Complement-type/LDLR Class A repeats that mediate ligand-binding and a cluster of six cysteine-poor LDLR Class B repeats (a.a. 495-714) that contains YWTD motifs involved in the pH-dependent ligands dissociation in endosomal compartments. The Class A and B clusters are separated by two EGF-like repeats (a.a. 356-391 and 396-431) and the Class B cluster is followed by an additional EGF-like repeat (a.a. 702-750). VLDLR and ApoER2 both function as receptor for reelin, a glycoprotein that is mainly secreted by Cajal Retzius neurons in the marginal zone and plays an essential role in the formation of the layered neocortex. Immunohistochemical staining reveals differential localization of VLDLR and ApoER2 in the developing mouse cerebral cortex, with VLDLR being localized to the distal portion of leading processes in the marginal zone (MZ) and ApoER2 mainly localized to neuronal processes and the cell membranes of multipolar cells in the multipolar cell accumulation zone (MAZ). The differential expression pattern may therefore allow distinct reelin actions on migrating neurons during both the early and late migratory stages in the developing cerebral cortex.

Specificity

Clone 4C4G5 (4G) detected VLDLR expression in embryonic and neonatal brain tissues from wild-type, but not Vldlr-knockout mice (Hirota, Y., et al. (2015). J. Comp. Neurol. 523(3):463-478).

Immunogen

Epitope: cytoplasmic domain

GST-tagged recombinant mouse VLDLR cytoplasmic tail (Hirota, Y., et al. (2015). J. Comp. Neurol. 523(3):463-478).

Application

Detect VLDLR using this rat monoclonal Anti-VLDLR, clone 4C4G5 Antibody, Cat. No. MABS1288, validated for use in Immunofluorescence and Western Blotting.

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected VLDLR expression in 4% paraformaldehyde-fixed, OCT-embedded frozen embryonic E17.5 mouse neocortex tissue section (Courtesy of Yuki Hirota, Ph.D., Keio University, Japan).

Immunofluorescence Analysis: A representative lot detected developmental stage-dependent VLDLR expression patterns among 4% paraformaldehyde-fixed, OCT-embedded frozen embryonic (E14.0 to E17.5) and neonatal (P0) brain tissues from wild-type, but not Vldlr-knockout mice (Hirota, Y., et al. (2015). J. Comp. Neurol. 523(3):463-478).

Western Blotting Analysis: A representative lot detected HA-tagged full-length mouse VLDLR exogenously expressed in HEK293T cells (Hirota, Y., et al. (2015). J. Comp. Neurol. 523(3):463-478).

Research Category
Signaling

Quality

Evaluated by Western Blotting in embryonic E16 mouse brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected VLDLR in 10 µg of embryonic E16 mouse brain tissue lysate.

Target description

~110 kDa observed. 93.49/96.37 kDa (mature/pro-form) calculated. The larger-than-calculated band size is consistent with that reported in the literature (Hirota, Y., et al. (2015). J. Comp. Neurol. 523(3):463-478). Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified.

Purified rat IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable