C5070
CHAPS hydrate
BioXtra, ≥98% (TLC)
Synonym(s):
3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
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About This Item
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description
zwitterionic
Quality Level
product line
BioXtra
assay
≥98% (TLC)
form
powder
mol wt
614.88 g/mol (anhydrous basis)
technique(s)
protein purification: suitable
protein quantification: suitable
impurities
≤0.001% Phosphorus (P)
≤0.1% Insoluble matter
<0.04% DMF
ign. residue
≤0.1%
mp
157 °C (dec.) (lit.)
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General description
Application
- 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
- 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
- HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition
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