C5070
CHAPS hydrate
BioXtra, ≥98% (TLC)
Synonym(s):
3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
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About This Item
Empirical Formula (Hill Notation):
C32H58N2O7S · xH2O
CAS Number:
Molecular Weight:
614.88 (anhydrous basis)
MDL number:
UNSPSC Code:
12161900
PubChem Substance ID:
NACRES:
NA.21
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description
zwitterionic
Quality Level
product line
BioXtra
assay
≥98% (TLC)
form
powder
mol wt
614.88 g/mol (anhydrous basis)
technique(s)
protein purification: suitable
protein quantification: suitable
impurities
≤0.001% Phosphorus (P)
≤0.1% Insoluble matter
<0.04% DMF
ign. residue
≤0.1%
mp
157 °C (dec.) (lit.)
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General description
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.
Application
- 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
- 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
- HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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