C5070

CHAPS hydrate

BioXtra, ≥98% (TLC)

• Synonym(s): 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
• Empirical Formula (Hill Notation): C₃₂H₅₈N₂O₇S · xH₂O
• CAS Number: 331717-45-4
• Molecular Weight: 614.88 (anhydrous basis)
• MDL number: MFCD00012116
• UNSPSC Code: 12161900
• PubChem Substance ID: 24892750
• NACRES: NA.21

Properties

• description: zwitterionic
• Quality Level: 400
• product line: BioXtra
• assay: ≥98% (TLC)
• form: powder
• mol wt: 614.88 g/mol (anhydrous basis)
• technique(s): protein purification: suitable; protein quantification: suitable
• impurities: ≤0.001% Phosphorus (P); ≤0.1% Insoluble matter; <0.04% DMF
• ign. residue: ≤0.1%
• mp: 157 °C (dec.) (lit.)

Description

General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

• 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
• 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
• HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable