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C5070

Sigma-Aldrich

CHAPS hydrate

BioXtra, ≥98% (TLC)

Synonym(s):

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS

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About This Item

Empirical Formula (Hill Notation):
C32H58N2O7S · xH2O
CAS Number:
331717-45-4
Molecular Weight:
614.88 (anhydrous basis)
MDL number:
MFCD00012116
UNSPSC Code:
12161900
PubChem Substance ID:
24892750
NACRES:
NA.21

description

zwitterionic

Quality Level

400

product line

BioXtra

assay

≥98% (TLC)

form

powder

mol wt

614.88 g/mol (anhydrous basis)

technique(s)

protein purification: suitable
protein quantification: suitable

impurities

≤0.001% Phosphorus (P)
≤0.1% Insoluble matter
<0.04% DMF

ign. residue

≤0.1%

mp

157 °C (dec.) (lit.)

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General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

  • 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
  • 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
  • HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCM2115
SLCL6111
SLCK8763
SLCG7375
SLCC6009

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