85878

Streptavidin from Streptomyces avidinii

affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein

• CAS Number: 9013-20-1
• MDL number: MFCD00082035
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• form: powder
• Quality Level: 200
• quality: affinity purified; lyophilized from 10 mM potassium phosphate
• specific activity: ≥13 U/mg protein
• mol wt: M_r ~60000
• solubility: H₂O: 1 mg/mL, clear to hazy, colorless to faintly yellow
• storage temp.: −20°C

Description

General description

Streptavidin is a crystalline protein synthesized by Streptomycetes.

Application

Streptavidin from Streptomyces avidinii has been used:

• in the pre-functionalization of self-propelled catalytic micromotors
• in the fabrication and modification of hydroxyapatite-chitosan (HA-CTS) nanofilm-coated substrates
• as an analyte to bind biomolecules to the gold-inverted polymer solar cells (Au-IPSC)

Biochem/physiol Actions

Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

The sales quantitites 1 mg and 5 mg

Unit Definition

1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5

Analysis Note

Binding capability: Streptavidin binds one molecule of biotin per subunit. Comparison of biotin binding and absorbance measurements indicates that ≤5% binding sites are occupied.

Other Notes

Review: Application of the avidin-biotin technique in microbiology; Protein isolated from the bacterium Streptomyces avidinii, having a high affinity for biotin; Avidin-Biotin technology

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)