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A4362

Sigma-Aldrich

Activin A human

Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE), suitable for cell culture

Synonym(s):

Erythroid differentiation protein, Inhibin beta A chain; Activin beta-A chain

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

Pricing and availability is not currently available.

Product Name

Activin A active human, Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)

biological source

human

Quality Level

recombinant

expressed in Nicotiana

assay

>97% (SDS-PAGE)

form

lyophilized

potency

≤5 ng/mL ED50

mol wt

27.4 kDa (disulfide-linked homodimers of two βA chains, each containing 116 amino residues)

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

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This Item
A4941B0939SRP3003
biological source

human

biological source

human

biological source

human

biological source

human

assay

>97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

>97% (SDS-PAGE)

assay

≥97% (HPLC), ≥97% (SDS-PAGE)

recombinant

expressed in Nicotiana

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in Nicotiana

recombinant

expressed in E. coli

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

-

form

lyophilized

form

lyophilized powder

form

lyophilized

form

lyophilized

UniProt accession no.

P08476

UniProt accession no.

P08476

UniProt accession no.

Q9Y275

UniProt accession no.

P08476

Amino Acid Sequence

HisHisHisHisHisHisGlyLeuGluCysAspGlyLysValAsnIleCysCysLysLysGlnPhePheValSerPheLysAspIleGlyTrpAsnAspTrpIleIleAlaProSerGlyTyrHisAlaAsnTyrCysGluGlyGluCysProSerHisIleAlaGlyThrSerGlySerSerLeuSerPheHisSerThrValIleAsnHisTyrArgMetArgGlyHisSerProPheAlaAsnLeuLysSerCysCysValProThrLysLeuArgProMetSerMetLeuTyrTyrAspAspGlyGlnAsnIleIleLysLysAspIleGlnAsnMetIleValGluGluCysGlyCysSer

General description

Activins are homodimers or heterodimers of the various β subunit isoforms, belonging to the TGFβ family. Mature Activin A has two 116 amino acids residues βA subunits (βA-βA). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells known to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells.
As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.

Physical form

Lyophilized from a Tris HCl 0.05 M buffer at pH 7.4

Reconstitution

Lyophilized protein should be reconstituted in water to a concentration of 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed.

Analysis Note

The biological activity of Activin A is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation.
ED50: ≤ 5 ng/mL

Other Notes

Extinction Coefficient: E0.1% = 1.27 (A 280 nm)
p.I: 7.27

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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