B0939

BAFF active human

Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)

• Synonym(s): B lymphocyte stimulator, B-cell-activating factor BAFF, BLyS, Dendritic cell-derived TNF-like molecule, TALL-1, TNF- and APOL-related leukocyte expressed ligand 1
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in Nicotiana
• assay: >97% (SDS-PAGE)
• form: lyophilized
• potency: ≤50 ng/mL ED₅₀
• mol wt: 18-20 kDa (a glycosilated polypeptide chain containing 151 amino acids)
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: Endotoxin, tested (LAL test, < 0.04 EU/ ug protein)

Description

General description

BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. It is also known as BLyS, THANK, TALL, zTNF4 and TNFS20. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C).The human BAFF gene code for a 285 amino acids type II transmembrane protein. Recombinant human soluble BAFF is a 151 amino acids containing the TNF-like portion of the extracellular domain of BAFF.

Physical form

Lyophilized from a 20 mM PBS buffer at pH 7 and 0.2 M NaCl.

Reconstitution

Lyophilized protein should be reconstituted in water to a concentration of 25 - 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed.

Analysis Note

The activity is determined by dose-dependeant stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method.
*activity results may vary with PBMC donors.
ED₅₀ ≤ 50 ng/mL

Other Notes

Extinction Coefficient: E^0.1% = 0.791 (A₂₈₀ nm)
p.I: 6.03

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable