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B0939

Sigma-Aldrich

BAFF active human

Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)

Synonym(s):

B lymphocyte stimulator, B-cell-activating factor BAFF, BLyS, Dendritic cell-derived TNF-like molecule, TALL-1, TNF- and APOL-related leukocyte expressed ligand 1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

Quality Level

200

recombinant

expressed in Nicotiana

assay

>97% (SDS-PAGE)

form

lyophilized

potency

≤50 ng/mL ED50

mol wt

18-20 kDa (a glycosilated polypeptide chain containing 151 amino acids)

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

Endotoxin, tested (LAL test, < 0.04 EU/ ug protein)

Related Categories

General description

BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. It is also known as BLyS, THANK, TALL, zTNF4 and TNFS20. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C).The human BAFF gene code for a 285 amino acids type II transmembrane protein. Recombinant human soluble BAFF is a 151 amino acids containing the TNF-like portion of the extracellular domain of BAFF.

Physical form

Lyophilized from a 20 mM PBS buffer at pH 7 and 0.2 M NaCl.

Reconstitution

Lyophilized protein should be reconstituted in water to a concentration of 25 - 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed.

Analysis Note

The activity is determined by dose-dependeant stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method.
*activity results may vary with PBMC donors.
ED50 ≤ 50 ng/mL

Other Notes

Extinction Coefficient: E0.1% = 0.791 (A280 nm)
p.I: 6.03

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Rita R Barbosa et al.
Journal of clinical immunology, 34(5), 573-583 (2014-05-09)
B-cell survival and differentiation critically depend on the interaction of BAFF-R and TACI with their ligands, BAFF and APRIL. Mature B-cell defects lead to Common Variable Immunodeficiency (CVID), which is associated with elevated serum levels of BAFF and APRIL. Nevertheless
Xiao-Juan Zhu et al.
British journal of haematology, 166(5), 783-791 (2014-06-04)
To investigate the expression of tumour necrosis factor superfamily 13B (TNFSF13B) receptors in immune thrombocytopenia (ITP) and their correlation with disease activity, we investigated the protein and mRNA levels of TNFSF13B, tumour necrosis factor receptor superfamily 13C (TNFRSF13C), TNFRSF13B and
Nuoyan Zheng et al.
BMC nephrology, 16, 72-72 (2015-05-16)
B cell activating factor belonging to the TNF family (BAFF) is vital for B cell survival, proliferation and activation. Evidence indicates that BAFF is systemically or locally increased in glomerulonephritis (e.g. lupus nephritis, IgA nephropathy). However, the effect of BAFF
J Wild et al.
Leukemia, 29(8), 1676-1683 (2015-02-25)
Natural killer (NK) cells are cytotoxic lymphocytes that substantially contribute to the therapeutic benefit of antitumor antibodies like Rituximab, a crucial component in the treatment of B-cell malignancies. In chronic lymphocytic leukemia (CLL), the ability of NK cells to lyse
Yoshiko Matsuda et al.
Cellular immunology, 295(2), 127-136 (2015-04-17)
Long-lived plasma cells (PCs) contribute to humoral immunity through an undefined mechanism. Memory B cells, but not human naïve B cells, can be induced to differentiate into long-lived PCs in vitro. Because evidence links a proliferation-inducing ligand (APRIL), a tumor
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