B1267

Biotin-maleimide

≥95% (TLC), powder

• Synonym(s): N-Biotinoyl-N′-(6-maleimidohexanoyl)hydrazide
• Empirical Formula (Hill Notation): C₂₀H₂₉N₅O₅S
• CAS Number: 116919-18-7
• Molecular Weight: 451.54
• MDL number: MFCD28397114
• UNSPSC Code: 12352203
• PubChem Substance ID: 329773179
• NACRES: NA.46

Properties

• Quality Level: 200
• assay: ≥95% (TLC)
• form: powder
• solubility: acetic acid: 20 mg/mL
• storage temp.: 2-8°C
• SMILES string: O=C(CCCCCN1C(=O)C=CC1=O)NNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23
• InChI: 1S/C20H29N5O5S/c26-15(7-2-1-5-11-25-17(28)9-10-18(25)29)23-24-16(27)8-4-3-6-14-19-13(12-31-14)21-20(30)22-19/h9-10,13-14,19H,1-8,11-12H2,(H,23,26)(H,24,27)(H2,21,22,30)/t13-,14-,19-/m0/s1
• InChI key: VAPZWEFPQRDVHR-NJSLBKSFSA-N

Description

General description

Biotin-maleimide is a biotin specific thiol containing reagent used to biotinylate different proteins through artificially induced or natural sulphadryl group. It can be used as a universal, multipurpose, thiol-specific probe. Biotin-maleimide can also be used in dot blots to detect protein SH groups.

Application

Biotin-maleimide- 0.01 mg/ml in PBS containing 0.1% Tween 20, incubated for 1 h at room temperature can be used to detect deprotected cysteine thiols present on immobilized peptides.

Sulfhydryl-specific biotinylation reagent. Couples by highly efficient thioether linkage. Typically used to couple to intrinsic sulfhydryls, e.g. protein, and avoid modification of primary amine.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)